Capcell Pak C18 MG | HPLC Column | Particle Size 5 µm

Capcell Pak C18 MG series focuses on "versatility" and seeks to achieve a balance between hydrophobicity and surface polarity, the application of which is extended to the analysis of chelating compounds by using metal-free filling technology and surface processing. This C18 HPLC column can be applied to a wide range of compounds from low to high polarity. 

Thanks to the polymer-coated base material and packing technology that take account of the influence of metallic impurities, CAPCELL PAK C₁₈ MG sharply elutes even compounds with highly coordinating properties like hinokitiol. Moreover, since the packing material has been designed to enhance surface polarity, it firmly retains even compounds with polarity sites like coordinate compounds.

Features

• From polar to hydrophobic compounds

Among C₁₈ columns, MG column presents an intermediate level of hydrophobicity and surface polarity. It is the recommended column for a wide range of substances, from polar to hydrophobic compounds.

For coordinate compounds in particular, peak tailing and absorption have been minimized to enable elution with sharp peaks.

• Coordinate compounds (hinokitiol) yet with a good peak profile

Thanks to the use of highly refined silica substrate and unique packing technology that minimized metallic impurity, the peaks of strong coordinate compounds like hinokitiol are eluted sharply. Because even trace amounts of metallic impurities greatly influence analysis results. Simply changing the column to MG will be your solution for absorption problem.

• Good lot-to-lot reproducibility

Excellent lot-to-lot reproducibility has been achieved by Osaka Soda's silica gel manufacturing technology and column packing technology.

Excellent lot-to-lot reproducibility has been achieved by Osaka Soda's silica gel manufacturing technology and column packing technology.

【HPLC Conditions】

Column	:	CAPCELL PAK C18 MG S2;2.0 mm i.d. x 50 mm
Mobile phase	:	10 mmol/L HCOONH4/CH3OH = 95 / 5
Flow rate	:	1 mL/min
Temperature	:	40℃
Detection	:	UV 254nm
Inj. vol.	:	1µL
Sample	:	1. Uridine 2. 2'-Deoxyuridine 3. Guanosine 4. 2'-Deoxyguanosine 5. 2'-Aminoadenosine 6. Adenosine (50 µg/mL each)

 

• With a particle size of 2μ, ultra-high-speed analysis is possible due to excellent separation performance.

Seven types of sulfa drugs were analyzed at ultra-high speed using CAPCELL PAK C₁₈ MG S2. The full analysis is done within 1 minute and all compounds achieve baseline separation.

【HPLC Conditions】

Column	:	CAPCELL PAK C18 MG S2;2.0 mm i.d. x 50 mm
Mobile phase	:	20 mmol /L Phosphate buffer (KH2PO4 : K 2HPO4 = 1 / 1 in molar ratio) / CH3OH = 80 / 20
Flow rate	:	900 µL/min
Temperature	:	40℃
Detection	:	UV 270nm
Inj. vol.	:	1µL
Sample dissolved in	:	Mobile phase ※ 1 µg/mL = 1 ppm
Sample	:	1. Sulfanilamide 2. Sulfamethizole 3. Sulfamerazine 4. Sulfapyridine 5. Sulfamethoxypyridazine 6. Sulfamethazine 7. Sulfaquinoxaline

Physical Properties

Functional group	Particle diameter (μm)	Pore diameter (nm)	Specific surface (m 2/g)	C%	Density (µmol/m 2)	pH range for use	USP
Octadecyl group	2	10	300	15	2.4	2～10	L1
Octadecyl group	3	10	300	16	2.6	2～10	L1
Octadecyl group	5	10	300	16	2.6	2～10	L1

 

Application Data

 Capcell Pak C18 MG Application Library