Capcell Pak C18 MG | HPLC Column | Particle Size 5 µm

Osaka SodaSKU: Sage-49

Inner Diameter (mm): 4.6 mm
Length (mm): 100 mm
Price:
Sale price$419.00 USD

Description

Capcell Pak C18 MG series focuses on "versatility" and seeks to achieve a balance between hydrophobicity and surface polarity, the application of which is extended to the analysis of chelating compounds by using metal-free filling technology and surface processing. This C18 HPLC column can be applied to a wide range of compounds from low to high polarity. 

Thanks to the polymer-coated base material and packing technology that take account of the influence of metallic impurities, CAPCELL PAK C18 MG sharply elutes even compounds with highly coordinating properties like hinokitiol. Moreover, since the packing material has been designed to enhance surface polarity, it firmly retains even compounds with polarity sites like coordinate compounds.


Features

  • From polar to hydrophobic compounds

Among C18 columns, MG column presents an intermediate level of hydrophobicity and surface polarity. It is the recommended column for a wide range of substances, from polar to hydrophobic compounds.

For coordinate compounds in particular, peak tailing and absorption have been minimized to enable elution with sharp peaks.

Hydrophobicity and surface polarity parameters of Osaka Soda columns

  • Coordinate compounds (hinokitiol) yet with a good peak profile

Thanks to the use of highly refined silica substrate and unique packing technology that minimized metallic impurity, the peaks of strong coordinate compounds like hinokitiol are eluted sharply. Because even trace amounts of metallic impurities greatly influence analysis results. Simply changing the column to MG will be your solution for absorption problem.

Fig. 1 : Peak profiles of a coordinate compound

  • Good lot-to-lot reproducibility

Excellent lot-to-lot reproducibility has been achieved by Osaka Soda's silica gel manufacturing technology and column packing technology.

Excellent lot-to-lot reproducibility has been achieved by Osaka Soda's silica gel manufacturing technology and column packing technology.

【HPLC Conditions】
Column : CAPCELL PAK C18 MG S2;2.0 mm i.d. x 50 mm
Mobile phase : 10 mmol/L HCOONH4/CH3OH = 95 / 5
Flow rate : 1 mL/min
Temperature : 40℃
Detection : UV 254nm
Inj. vol. : 1µL
Sample : 1. Uridine
2. 2'-Deoxyuridine
3. Guanosine
4. 2'-Deoxyguanosine
5. 2'-Aminoadenosine
6. Adenosine (50 µg/mL each)

 

  • With a particle size of 2μ, ultra-high-speed analysis is possible due to excellent separation performance.

Seven types of sulfa drugs were analyzed at ultra-high speed using CAPCELL PAK C18 MG S2. The full analysis is done within 1 minute and all compounds achieve baseline separation.

図2サルファ剤の超高速分析例

【HPLC Conditions】
Column : CAPCELL PAK C18 MG S2;2.0 mm i.d. x 50 mm
Mobile phase : 20 mmol /L Phosphate buffer (KH2PO4 : K 2HPO4 = 1 / 1 in molar ratio) / CH3OH = 80 / 20
Flow rate : 900 µL/min
Temperature : 40℃
Detection : UV 270nm
Inj. vol. : 1µL
Sample dissolved in : Mobile phase
※ 1 µg/mL = 1 ppm
Sample : 1. Sulfanilamide
2. Sulfamethizole
3. Sulfamerazine
4. Sulfapyridine
5. Sulfamethoxypyridazine
6. Sulfamethazine
7. Sulfaquinoxaline


Physical Properties

Functional group Particle
diameter
(μm)
Pore
diameter
(nm)
Specific
surface
(m 2/g)
C% Density
(µmol/m 2)
pH range
for use
USP
Octadecyl group 2 10 300 15 2.4 2~10 L1
Octadecyl group 3 10 300 16 2.6 2~10 L1
Octadecyl group 5 10 300 16 2.6 2~10 L1

 

Application Data

 Capcell Pak C18 MG Application Library

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