|HIRANUMA APPLICATION DATA||Automatic Titrator||Data No.||A7||Apr. 19,2018|
|FOOD||Acid value of cooking oil|
It is used for evaluation of free fatty acid content as quality index of oils and fats. This method is described in a variety of official standards such as “Japan Agricultural Standards” and Pharmacopoeias. Example of titration for acid value in cooking oil is introduced here.
Reference: 1) Japanese Pharmacopoeia Seventeenth Edition
2. Configuration of instruments and reagents
|Main unit||:||Hiranuma Automatic Titrator COM series|
|Electrode||:||Glass reference combination electrode GR-522BZ, Connect to IE-1|
|Components||:||Buret tip (Tube Type), tubing accessories|
|Titrant||:||0.1 mol/L Pottasium hydroxide ethanolic standard solution|
|Titration solvent||:||Mixed solvent of ethanol and diethyl ether with 1 : 1 ratio [v/v]|
3. Measurement procedure
|(1)||Take 20 g* of sample into 200 ml Erlenmeyer flask and weigh it exactly.|
|(2)||Add 100 ml of titration solvent and dissolve the sample.|
|(3)||Immerse the electrode and start titration.|
|(4)||Blank measurement is also performed with procedure (2) – (3).|
|(5)||After titration finished, wash electrode with solvent and then immersed into DI water for 5 minutes to maintain response of glass electrode.|
|※||Sample size depends on expected value, and it’s described in a standard method.|
4. Measurement conditions and results
Examples of titration conditions
Measurement of blank
Measurement of sample
|BLANK||1||–||0.013||–||Avg. 0.013 mL|
|SAMPLE||1||20.2103||3.075||0.850||Avg. 0.839 mgKOH/g|
|2||20.0897||3.051||0.848||SD 0.017 mgKOH/g|
|3||20.0007||2.936||0.820||RSD 2.00 %|
Examples of measurement curves
|(1)||Maintenance of electrode
As glass electrodes continue to be used in nonaqueous solvents, sensitivity decreases. To recover the sensitivity of glass electrode, immerse the electrode into DI water for 5 minutes after each measurement. Similarly, when reference electrode is used in a nonaqueous solvent, KCl tend to crystallize on the sleeve part of reference electrode. Crystals of KCl can be easily washed off with water.
|(2)||Method of endpoint detection
In this report, titration curve shows clear inflection point as endpoint. When the sample is used oil, inflection points may be unclear and cannot be detected. In that case, endpoint could be defined as fixed pH value within indicator range for pH indicator reagent. Parameters “Method” could be set to “SET” to detect endpoint with fixed pH. And parameter “Endpoint pH” also could be set to defined pH value.
Keywords: Cooking oil, Acid value, Pharmacopoeia
*Some measurement would not be possible depending on optional configuration of system.