The 15ml centrifugal filter devices provide rapid and efficient concentration and purification of up to 15.0 ml of biological samples. The unique vertical design and maximum filtration area provide fast sample processing and high sample recovery rate (typically greater than 90% of dilute starting solution), while maintaining a gentle concentration environment to preserve protein activity and conformation. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical dead-stop in the filter device prevents spinning to dryness and potential sample loss. The wide selection of ultra filtration molecular weight cut-off (MWCO) devices incorporate PES membrane, which is very low in protein and nucleic acid binding.
Features
- Low biological binding and high chemical compatibility
- Special design to prevent drying, which can avoid damage to the samples due to excessive centrifugation
- Optional pore sizes for various MWCO requirements
- Effective filtration area up to 7.2cm2, larger area allows higher flowrate
- 100% pass integrity testing
Application
- Concentration/Desalination of Proteins/Nucleic Acids;
- Buffer Exchanging or Chromatography Component Desalting;
- Bio-molecules Harvesting from Culture Media;
- Concentration/Separation of Viruses;
- Coarse Separation of Bio-molecular Mixtures;
- Removal of Particles and Debris from Celllysate;
- Removal of Primers, Adapters, or Molecular Markers from Reaction mixtures.
Specifications
Material & Size
| Filter |
PC Material |
| Membrane |
PES membrane |
| Filtrate tube |
PP material |
| Filtrate cap and liner |
PP material |
| Filters in test tubes (capped) |
Overall length 117mm, Diameter 31mm |
| Filter |
Length: 75 mm, Diameter:28 mm |
Capacity:
| Effective filter area |
7.2 cm2 |
| Maximum sample volume |
15mL |
| Maximum filtrate volume |
17mL |
| Retention volume |
20μL |
| Locking volume |
300μL |
| Operating temperature |
0-40°C |
| pH range |
1-14 |
| Maximum centrifugal force: |
Swing bucket rotor 4000x g |
| Fixed angle rotor 5000x g |
Order Information:
| Article No |
Description |
Qty/pk |
| UTPES150005N |
15ml, PES, 5KD, red |
12 pcs |
| UTPES150010N |
15ml, PES, 10KD, blue |
12 pcs |
| UTPES150030N |
15ml, PES, 30KD, yellow |
12 pcs |
| UTPES150050N |
15ml, PES, 50KD, orange |
12 pcs |
| UTPES150100N |
15ml, PES, 100KD, white |
12 pcs |
Precautions
- The 15mL ultra-filtration centrifuge tube is non sterilized disposable product. If it is damaged, please do not use it.
- The 15mL ultra-filtration centrifuge tube is only for research use, not for in vitro diagnosis.
- To ensure the suitability of the sample, it is recommended to conduct a pre experiment for the first use to test the recovery and retention efficiency.
*To achieve the highest recovery, MWCO membrane selected should be at least half the molecular weight of the solute to be retained.
