Primaide 1440 Fluorescence Detector | Hitachi


High-Performance Liquid Chromatography (HPLC) is utilized in a wide range of fields, such as medicine, food production, chemistry, and environmental science, making the demand for HPLC systems higher among the various types of analytical equipment. The Hitachi Primaide HPLC is designed for long-term, stable operation, and features both high reliability and superior durability. It offers tremendous capability for everyday analysis.

The Primaide 1440 FL detector has a wide range of wavelength setting and high sensitivity. The front access layout makes easy maintenance possible, same as the Primaide 1410 UV detector.

Primaide HPLC System Features 

  • Optional units mounted inside provide clean appearance
    All principal options, including a degasser, gradient unit, and sample cooling system can be mounted inside the main body. It is not necessary to make room for optional units.
  • Front access layout for improved operation and maintenance
    Every module is accessed and handled from the front. Since connections are arranged on the front, it is easy to replace columns and manipulate the flow system. For easier maintenance, the lamp and cell unit are also easily accessible.
  • Leak sensors in all modules
    Any leakage in the flow system is detected by leak sensors built into each module. After detection the system halts, ensuring improved safety and reliability.
  • Complete control with system manager
    A control panel is not necessary, as everything can be controlled via the system manager. By installing a UI- pad (optional), each module can be controlled separately.
  • e-Line and USB
    The e-Line is an advanced version of the existing D-Line. All modules are connected by an e-Line in a single chain, and all the communications are integrated. The use of digital signals improves the reliability of data transactions. The interface between the PC and equipment is via versatile USB. A wide range of PCs are supported, enabling flexible configuration.

Primaide 1440 FL Detector Features

  • Increased sensitivity with optical system that keeps light loss to a minimum
    The Primaide 1440 FL Detector is a bright optical system which has attained a S/N greater than 900 (by baseline method, Em spectrum band width: 30 nm) versus a Raman peak of water. Signal-to-Noise ratio (S/N) refers to the ratio between the peak intensity and baseline noise. A good S/N is necessary to detect trace amounts of components. A reduced detector noise improves sensitivity.
  • Automatic wavelength calibration
    Wavelength calibration is automated by the built-in Hg (mercury) lamp.
  • Selectable slit
    The slit of the emission side monochromator is selectable from 15 nm and 30 nm. This allows analysis with a higher sensitivity.
  • Front panel access for easy maintenance
    User maintenance (such as positional adjustment of Xe lamp, flow cell replacement, etc.) is facilitated through front panel access to the instrument.

    Hitachi Primaide 1410 Detector Lamp Replacement from JM Science

SpecificationsHitachi Primaide 1440 FL Detector from JM Science Inc.

Additional Resources

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Application Library

 Analysis of Catechins

Catechins are important antioxidant components found in tea. Due to their antioxidant properties and wide range of efficacy, research of catechins has been receiving an increasing amount of attention. The catechin profile in tea has been found to differ depending on the method of tea preparation. In addition, the catechins in tea form thermal isomers during the sterilization process of commercially available bottled tea drinks. The concentration of thermal isomers reaches significant levels, and, as such, the physiological function of these isomers is also of interest. The following describes examples of analysis of catechins and caffeine in tea.


 Analysis of Organic Acids using UV Detection

Organic acids are known as ingredients that determine the taste and flavor of foods. In addition to the food sector, many other areas such as pharmaceutical and chemical industries, environmental analysis, and biotechnology, analyze organic acids.
Analysis methods of organic acids include UV detection (detection of absorption of carboxyl groups), electrical conductivity detection (detection of ionic compounds), a method using pH indicators (visible absorption detection of pH changes caused by acidic components) and a post-column derivatization method for high selectivity of detection. An appropriate method must be selected based on analysis samples and purposes.
The following describes examples of analysis of organic acids using a portable UV detection system and the reversed-phase column, "LaChrom C18-AQ".


 Simultaneous Analysis of Phenoxyethanol and Parabens

Paraben is a generic term for p-hydroxybenzoate esters. Due to their low toxicity in the human body, as well as antiseptic and mold proofing effects, parabens are used as preservatives in medicines, cosmetics, and foods. Phenoxyethanol is another component used as a germicide and antiseptic in cosmetics. It is a naturally-occurring compound that is found in substances such as green tea.
Phenoxyethanol is often used in combination with parabens in cosmetics to reduce the added paraben content.
In this presentation, we will introduce and examine the simultaneous analysis of phenoxyethanol and parabens.


 Analysis of Artificial Sweeteners

Aspartame is an artificial sweetener that is 100 to 200 times sweeter than sucrose. It is a dipeptide formed by peptide bonding of methyl ester of phenylalanine with aspartic acid. Aspartame is mainly added to low calorie and non-caloric drinks and foods. It is also sometimes used with other sweeteners such as sugar and sorbitol.
Examples of analyses for aspartame in (1) carbonated drink and (2) apple cider vinegar drink are introduced here.

These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.

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