The Capcell Pak C18 AQ is a high-polarity C18 column with durability that has been improved using Osaka Soda's original polymer coating technology. In addition to the stability in 100% aqueous mobile phase, the polymer coating minimizes the influence of residual silanol groups and provides good peak shape for basic compounds.
Stable reproducibility with 100% aqueous mobile phase
Although retention can be increased by lowering the rate of organic solvent in the mobile phase with the C18 column, reproducibility cannot be realized with 100% aqueous mobile phase.
CAPCELL PAK C18 AQ addresses this by assuring good reproducibility even with 100% aquatic moble phase by optimizing the introduction of octadecyl group and polymer coating of the base packing material with a novel polymer.
The highest surface polarity among C18 columns
With the highest surface polarity among C18columns, the AQ type enables strong retention and high separation of high polarity compounds. Furthermore, it enables the use of 100% aqueous mobile phase, which used to present problems in reproducibility with the conventional ODS columns.
Additionally, since it is coated with a polymer, the peak profile of basic compounds is free from tailings and, consequently, extremely good.
Excellent retention capacity for high polarity compounds
Designed for high surface polarity, CAPCELL PAK C18 AQ is the only column among CAPSELL PAK products that enables stable analysis with 100% aqueous mobile phase. Moreover, it has excellent retention capacity for high polarity compounds.
|Column:||CAPCELL PAK C18 AQ S3|
|Mobile phase:||10mmol/L CH3COONH4|
|Sample:||(1) Nicotinic acid|
|(Concentration is 100μL/mL for all)|
Higher-than-expected separation capacity even with 100% organic solvent mobile phase
Since the type of polymer coating and the introduction rate of C18 have been modified to enable the use of 100% aqueous mobile phase, hydrophobic compounds can be eluted faster with 100% organic mobile phase.
High resistance to acid and alkali
Poor durability is a problem in columns designed for use with 100% aqueous mobile phase. However, the AQ has been designed to improve resistance to acid and alkali with a polymer coating and the introduction of a unique C18 group.
AQ shows excellent resistance to acid and alkali in comparison with other columns capable of using 100% aqueous mobile phase, in which the resistance tends to deteriorate quickly.
Test conditions for acid resistance
Test conditions for alkali resistance
Good peak profile also with basic compounds
The AQ type column comprises packing material coated with polymer; least influenced by silanol, and, consequently, is able to elute the basic compounds as a sharp peak without tailing.
Column: 4.6mm i.d.×150mm
Realization of low pressure allows a length of 250 mm and a particle diameter of 3 µm
Through the selection of silica gel accurately graded for AQ, high number theory plate (NTP) in 5 and 3 µm particle with an amazingly low pressure became available.
The changeover from a particle of 5 µm to 3 µm has enabled improved separation and fast analysis. Which option would you prefer to pursue in the analysis of high polarity compounds?
Conditions for comparison of column pressure